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Cavaco, M., Valle, J., Flores, I., Andreu, D. & Castanho, M. A. R. B. (2021). Estimating peptide half-life in serum from tunable, sequence-related physicochemical properties. Clinical and Translational Science, 14(4), 1349?1358. https://doi.org/10.1111/cts.12985

M. Cavaco et al.,  "Estimating peptide half-life in serum from tunable, sequence-related physicochemical properties", in Clinical and Translational Science, vol. 14, no. 4, pp. 1349-1358, 2021

@article{cavaco2021_1790983797894,
	author = "Cavaco, M. and Valle, J. and Flores, I. and Andreu, D. and Castanho, M. A. R. B.",
	title = "Estimating peptide half-life in serum from tunable, sequence-related physicochemical properties",
	journal = "Clinical and Translational Science",
	year = "2021",
	volume = "14",
	number = "4",
	doi = "10.1111/cts.12985",
	pages = "1349-1358",
	url = "https://ascpt.onlinelibrary.wiley.com/journal/17528062"
}

TY  - JOUR
TI  - Estimating peptide half-life in serum from tunable, sequence-related physicochemical properties
T2  - Clinical and Translational Science
VL  - 14
IS  - 4
AU  - Cavaco, M.
AU  - Valle, J.
AU  - Flores, I.
AU  - Andreu, D.
AU  - Castanho, M. A. R. B.
PY  - 2021
SP  - 1349-1358
SN  - 1752-8054
DO  - 10.1111/cts.12985
UR  - https://ascpt.onlinelibrary.wiley.com/journal/17528062
AB  - Proteolytic instability is a critical limitation for peptide-based products. Although significant efforts are devoted to stabilize sequences against proteases/peptidases in plasma/serum, such approaches tend to be rather empirical, unspecific, time-consuming, and frequently not cost-effective. A more rational and potentially rewarding alternative is to identify the chemical grounds of susceptibility to enzymatic degradation of peptides so that proteolytic resistance can be tuned by manipulation of key chemical properties. In this regard, we conducted a meta-analysis of literature published over the last decade reporting experimental data on the lifetimes of peptides exposed to proteolytic conditions. Our initial database contained 579 entries and was curated with regard to amino acid sequence, chemical modification, terminal half-life (t1/2) or other stability readouts, type of stability assay, and biological application of the study. Although the majority of entries in the database corresponded to (slightly or substantially) modified peptides, we chose to focus on unmodified ones, as we aimed to decipher intrinsic characteristics of peptide proteolytic susceptibility. Specifically, we developed a multivariable regression model to unravel those peptide properties with most impact on proteolytic stability and thus potential t1/2 predicting ability. Model validation was done by two different approaches. First, a library of peptides spanning a large interval of properties that modulate stability was synthesized and their t1/2 in human serum were experimentally determined. Second, the t1/2 of 21 selected peptides approved for clinical use or in clinical trials were recorded and matched with the model-estimated values. With both approaches, good correlation between experimental and predicted t1/2 data was observed.
ER  -