Exportar Publicação

A publicação pode ser exportada nos seguintes formatos: referência da APA (American Psychological Association), referência do IEEE (Institute of Electrical and Electronics Engineers), BibTeX e RIS.

Exportar Referência (APA)
Duarte, I. C., Ricardo, C. P. P. & Duque-Magalhães, M. C. (1996). Proteolysis in the quiescent seed. Physiologia Plantarum. 96 (3), 519-525
Exportar Referência (IEEE)
I. C. Almeida et al.,  "Proteolysis in the quiescent seed", in Physiologia Plantarum, vol. 96, no. 3, pp. 519-525, 1996
Exportar BibTeX
@article{almeida1996_1732355922158,
	author = "Duarte, I. C. and Ricardo, C. P. P. and Duque-Magalhães, M. C.",
	title = "Proteolysis in the quiescent seed",
	journal = "Physiologia Plantarum",
	year = "1996",
	volume = "96",
	number = "3",
	doi = "10.1111/j.1399-3054.1996.tb00467.x",
	pages = "519-525",
	url = "http://onlinelibrary.wiley.com/doi/10.1111/j.1399-3054.1996.tb00467.x/full"
}
Exportar RIS
TY  - JOUR
TI  - Proteolysis in the quiescent seed
T2  - Physiologia Plantarum
VL  - 96
IS  - 3
AU  - Duarte, I. C.
AU  - Ricardo, C. P. P.
AU  - Duque-Magalhães, M. C.
PY  - 1996
SP  - 519-525
SN  - 0031-9317
DO  - 10.1111/j.1399-3054.1996.tb00467.x
UR  - http://onlinelibrary.wiley.com/doi/10.1111/j.1399-3054.1996.tb00467.x/full
AB  - The importance of the proteolytic system of the quiescent seed in regard to mobilization of storage protein was assessed by identification of proteases already present at this stage. Extracts of quiescent cotyledons of white lupin (Lupinus albus) submitted to 45 h autolysis in vitro displayed a higher degree of protein degradation than that observed in vivo after two days of seed imbibition. Differences in the susceptibility to proteolysis were verified by densitometric analysis of the polypeptides after electrophoretic separation. The pH dependence of the proteolytic activities and the responses to specific protease inhibitors showed that the proteolytic systems vary from quiescent to 1 - to 3-day-imbibed cotyledons. By labelling an endogenous globulin with 125I a sensitive radiometric assay allowed the identification of both an acidic and a neutral proteolytic system in the quiescent cotyledon. Within the quiescent seed there already exists a high potential for initiating proteolysis, so that the requirement for proteolysis by specific endopeptidases synthesized de novo upon imbibition only applies to part of the reserve proteins.
ER  -